STEPS FOR PROCEDURES
  • HARVESTING SKIN GRAFT
    • Try to take only epidermis this prevents
      • The scaring
      • Fast healing of donor area
      • Decreases the dissection time
    • Preferably graft is taken from hidden areas.(buttocks,thighs)
    • One tenth the size of recipient area
  • CELLULAR SEPERATION
  • • The epidermal layer is incubated in 0.25% trypsin EDTA, solution, in a petri dish, for 45min-60min
    • The dermal surface should be kept above
    • This petridish is now placed in a Co2incubator at 370c, 5% Co2, for 50 minutes
    • The epidermal sheets are gently manipulated with forceps to dissociate the epidermal cells
    • Residues of dermis are discarded
    • Patients serum about 0.5-1ml is added to neutralize trypsin
    • The cells are washed with DMEM solution, centrifuged for 5 min at 1500rpm
    • Supernant solution is discarded and the cellular pallet is mixed with patients serum (1-2ml)
    • This is filled in insulin syringes, and is ready for transplant

  • TRANSPLANTATION
  • Microdermabrasion is done with laser or micromotor
    • Can be done under G.A, or LA
    • Only the epidermis is removed
    • Cells are sprinkled over the site and kollagen dressing is given
    • Part is kept immobilized for 6 hrs

  • POST OPERATIVE
  • • Dressing is opened on fifth day.
    • Initially red discolouration will be seen.
    • In 3-6 months normal colour appears.
    • Excellent cosmetic results are seen in 5-6 months.
    • There is no side effect after the procedure.
    • Some patients may require more than one sitting for 100% pigmentation